Chemical constituents and bioactivity of Codium amplivesiculatum setchell & N. L. Gardner (Chlorophyta; Bryopsidales)
DOI:
https://doi.org/10.37543/oceanides.v28i2.124Keywords:
1-octodecanol, clerosterol, anti-tuberculosis, fatty alcoholAbstract
In search of bioactive substances from Mexican marine organisms, crude ethanol-extract from
the marine alga Codium amplivesiculatum was fractionated in chromatographic columns of silica gel at 60 Å (230-400 mesh) using solvent mixtures of increasing polarity. All the fractions were submitted to antibacterial assays. The major metabolite from an anti-tuberculosis fraction (MIC = 100 µg mL–1) was purified and identified as 1-octodecanol (1). The anti-tuberculosis activity was attributed to 1 with bases in previous reports. In addition, clerosterol (2) was obtained by crystallization from an active fraction against Staphylococcus aureus and Vibrio parahaemolyticus (MIC = 125 and 250 µg mL–1, respectively). Both structures were established by interpretation and comparison of infrared and 1H NMR spectroscopic data. In contrast with other studies, 2 showed a nonsignificant cytotoxicity against the cell line PC-3 (% GI = 21.05 ± 0.3 at 50 µg mL–1). To our knowledge, these metabolites are reported for the first time from C. amplivesiculatum, and this is one of very rare reports of saturated long-chain alcohols isolated from chlorophytes.
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