Protocol optimization for DNA extraction and amplification by PCR of denitrifying organisms.
DOI:
https://doi.org/10.37543/oceanides.v29i2.138Keywords:
denitrifying microorganisms, molecular markers, environmental DNA extractionAbstract
In this contribution we had the objective to solve the problems encountered working with molecular
biology techniques for the extraction of DNA obtained from marine suspended particulate matter, and we provide an alternative on how to resolve such problems. Commonly employed protocols were modified to optimize the
total DNA extracted increasing in terms of quality and quantity the probability of amplifying the functional genes. In addition, the results of the selection of hypervariable sites of 16S rARN and nirS, nirK and nosZ genes are presented for the determination of amplicons useful in the identification of microorganisms participating in the denitrification pathway in the water column.
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