Preliminary phytochemical and biological activity screening of Sargassum lapazeanum: Biological activity of S. lapazeanum

José  Manuel Mendoza Álcala; Mauricio Muñoz Ochoa; Yoloxochitl Elizabeth Rodríguez Montesinos; Dora Luz Arvizu Higuera

doi:10.37543/oceanides.v39i1.284

Authors

José Manuel Mendoza Álcala
Mauricio Muñoz Ochoa CICIMAR
Yoloxochitl Elizabeth Rodríguez Montesinos CICIMAR
Dora Luz Arvizu Higuera CICIMAR

DOI:

https://doi.org/10.37543/oceanides.v39i1.284

Keywords:

antioxidant, flavonoids, hemolytic, toxicity, triterpenes

Abstract

Algae are exposed to substantial stress. In response, these organisms have developed efficient defense systems, such as protective secondary metabolite synthesis, making algae a primary source of bioactive compounds with a wide spectrum of biological activities. Thus, algae show potential for use in treatments of thrombotic, infectious, and chronic degenerative diseases. Therefore, the objective of this study was to evaluate the phytochemical compounds and pharmacological activity of an extract obtained from Sargassum lapazeanum. Algae were collected in the intertidal zone of Tarabillas beach (Bahía de La Paz, BCS). The biological activities of an ethanolic extract and its fractions were evaluated using chromatographic techniques. In addition, a bioautographic assay of hemolytic activity was conducted, and phytochemical profiles and acute toxicity in Artemia franciscana were evaluated. The relationships among the main extract components were also determined. The ethanolic extract exhibited significant antioxidant and hemolytic activity, which was mainly attributed to its content of anthrones, anthraquinones, and unsaturated triterpenes. Its toxicological activity reached an LC₅₀ value of 225.1 μg mL^-1, which was mainly attributed to alkaloids, flavonoids, anthrones, and saponins. The results suggest that Sargassum lapazeanum, which is endemic to the Gulf of California, has great pharmacological potential with biomedical applications.

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Author Biographies

José Manuel Mendoza Álcala

Es exalumno y no tenemos ninguna referencia de su paradero

Mauricio Muñoz Ochoa, CICIMAR

Obtained his Bachelor’s in Biochemical Engineering (1997) from the Instituto Tecnológico de la Paz (ITLP) and his Master's (2004) and Doctorate (2007) from Centro Interdisciplinario de Ciencias Marinas of Instituto Politécnico Nacional (CICMAR). His research focuses on chemical ecology and natural marine products. He is currently conducting research on the use of algae as bioindicators of pollution and sources of bioactive (antibacterial, antioxidant, cytotoxic, and anticoagulant) compounds. He is the author of 23 scientific publications, 3 book chapters, and 2 popular articles. He has directed 4 Bachelor’s theses, 11 Master's theses, and 3 Doctorate theses. He is a member of the National System of Researchers (level 1).

Yoloxochitl Elizabeth Rodríguez Montesinos, CICIMAR

Obtained her Bachelor’s in Marine Biology (1989) from the Universidad Autónoma de Baja California Sur (UABCS) and her Master’s degree (1998) from the Centro Interdisciplinario de Ciencias Marinas of Instituto Politécnico Nacional (CICMAR). The research that she has conducted has focused on the study and production of algal polysaccharides of commercial interest and on the search for bioactive compounds from algae. She has published 30 scientific articles and 2 popular science articles. She has directed 1 Bachelor’s thesis and 2 Master's theses.

Dora Luz Arvizu Higuera, CICIMAR

Obtained here Bachelor’s degree in Biochemical Engineering (1988) from the Instituto Tecnológico de la Paz (ITLP) and her Master’s degree in Marine Sciences (1993) from the Centro Interdisciplinario de Ciencias Marinas del Instituto Politécnico Nacional (CICIMAR). Her research focuses on the production of algal polysaccharides for commercial use and on the search for bioactive compounds from algae. She has published 22 scientific articles and directed 1 Master's thesis. She currently participates in the Biotechnology Network of the Instituto Politécnico Nacional.

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